FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500339
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64090.4271701262181414No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA54630.3641177093976761No Hit
CTCCTGCACGGCCAGTCAGAGTGTTGCCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACT45290.3018651118180624No Hit
GTATAATCACTGGTTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40920.2727383611303846No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39050.26027451129378093No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT37870.25240962209207385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37370.24907704192185898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33880.22581563233375923No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT32000.21328513089375134No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31790.2118854472222611No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA31700.2112855827916224No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC31680.21115227958481386No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC30080.20048802304012628No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT29970.19975485540267898No Hit
GTGATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT29870.199088339368636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29640.19755535249033718No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29430.19615566881884694No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTGTTGCCAC28540.1902236761158645No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28400.18929055366820433No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA28130.1874909603762883No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTGTTGCCACCAACTTAGCCTGGTA26050.17362742686819446No Hit
GTAATACACGGCTGTGTCCGCGGCAGTCACGGAGCTCAGGTTCAGGGAGA25510.1700282402843624No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25300.16862855661287215No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23500.15663126800009866No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23190.15456506829456543No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23150.15429846188094826No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT22800.15196565576179782No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGGTGGCA22110.14736669512690131No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21900.14596701145541108No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21440.1429010376988134No Hit
GTCAGAGTGTTGCCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTG21100.1406348831830673No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC21010.1400350187524286No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAATTACCACTGGCACTGGATCCG20840.13890194149455556No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA20330.1355027097209364No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20330.1355027097209364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20010.1333698584119989No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19920.13276999398136022No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19560.13037053625880551No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA18930.12617148524433477No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18620.12410528553880157No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18590.12390533072858868No Hit
GTTTATTACTGTCAGCAGTATAATCACTGGTTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17760.118373247646032No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG17590.11724017038815895No Hit
ACCCTCTCCTGCACGGCCAGTCAGAGTGTTGCCACCAACTTAGCCTGGTA17440.11624039633709449No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT17320.11544057709624292No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG17070.11377428701113548No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16890.11257455814985813No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16770.11177473890900656No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG16650.110974919668155No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16620.1107749648579421No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16170.10777564270474872No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16170.10777564270474872No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16130.10750903629113155No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15870.10577609460261982No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15580.1038431981038952No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15550.1036432432936823No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT15300.10197695320857485No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA15190.10124378557112759No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15160.1010438307609147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33050.041.4328651
GGTATCA13450.041.0320931
TATCAAC37700.036.2019422
ATCAACG39150.034.8297463
TCAACGC39250.034.7305834
GTATAGA1003.8198777E-1034.4926031
CAACGCA40300.033.8256955
AACGCAG41850.032.6551486
AGAGTAC43400.031.33652311
CGCAGAG43700.031.1939498
ACGCAGA44900.030.4369247
GCAGAGT46000.029.634259
CAGAGTA47750.028.47893510
AGTACGG37000.026.24338213
GAGTACT14800.025.82460212
TAGTACG809.708908E-525.8177194
GAGTACG37900.025.71103912
TATAAGA2050.025.2182792
ACGGGAT4450.024.7664516
GTATGAT700.001335703224.6375711
Produced by FastQC (version 0.11.5)