FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314573
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37300.038.309121
GGTATCA16700.037.730931
ATCAACG43850.032.502073
TCAACGC44150.032.1252754
CAACGCA45250.031.268255
TATCAAC45850.031.2344822
AACGCAG46450.030.4592976
GTGGTAT6600.029.2150751
ACGCAGA50600.027.8261477
TCGTATG756.000817E-527.7090440
TACCGTA500.0078173427.5404037
CGCAGAG52150.027.0651158
AGAGTAC51400.026.99120311
TATTACG658.7327324E-426.499326
CAGAGTA52550.026.40052810
TGGTATC7200.026.297262
GCAGAGT55350.025.5003749
CGAATTA700.001350556524.5905815
CTCGTAT851.4126554E-424.43045439
GAGTACG42550.023.22002212
Produced by FastQC (version 0.11.5)