FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314573
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14550.11068232802590651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42050.040.2961771
GTGGTAT8050.038.932321
TCAACGC48400.034.7901464
ATCAACG48700.034.66093
CAACGCA49200.034.433995
TGGTATC9000.034.0302162
AACGCAG50000.033.9517756
TATCAAC50700.033.462332
ACGCAGA53700.031.6764457
ACGGTAT800.00434397431.05744470
CGCAGAG55250.030.7877868
GGTATCA17400.030.4814931
AGAGTAC54900.030.36274311
CAGAGTA55450.029.9938910
GCAGAGT58550.028.9340389
GAGTACT20450.024.87462612
AGTACTT20700.024.7411913
AGTACGG47000.024.71857813
GAGTACG47150.024.56610912
CGAACCT4050.023.29488853
Produced by FastQC (version 0.11.5)