FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178259
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCGGCAGTCACAGAGCTCAGGTGCAGGGAGA12110.10277876086666853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11960.1015056961160492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24100.038.399891
GTGGTAT4650.037.8729741
GGTATCA10900.037.3824461
TATCAAC28100.033.2953072
ATCAACG28650.032.5356253
TCAACGC29000.032.1429564
CAACGCA29600.031.724685
AACGCAG29750.031.5647226
TGGTATC5750.031.2216282
AGAGTAC33300.027.8851411
ACGCAGA33750.027.8201777
CGCAGAG34550.027.0760928
CAGAGTA34500.027.01527810
CGCGTAT658.628677E-426.55347812
GCAGAGT36850.025.4798099
GTAGTTT3750.024.58674670
AGTACGG29900.023.55177913
GAGTACG30200.023.31782212
GTCTATA1052.2649361E-523.020831
CCGCGTA750.001986507823.01301411
Produced by FastQC (version 0.11.5)