FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178259
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12380.10507027741778335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.039.923981
GTATCAA30250.038.466851
TGGTATC6750.035.7848742
TATCAAC32900.035.3459132
GGTATCA12950.034.9287951
ATCAACG34300.033.5728843
TCAACGC34350.033.5126344
CAACGCA34900.032.9830975
AACGCAG35300.032.6093526
ACGCAGA38450.029.9378437
AGAGTAC38700.029.83603311
CAGAGTA39150.029.40255710
CGCAGAG39650.029.031788
GCAGAGT41050.028.1256169
TTCTGAT8800.024.96148970
AGTACGG34800.024.77045413
TAGCGTG700.001341920224.617415
GAGTACG35450.024.31110812
TATAAAG1952.6735815E-522.52934570
GTATTAA1304.8864695E-621.2485221
Produced by FastQC (version 0.11.5)