FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439326
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45150.038.438491
GGTATCA19150.034.6354831
TATCAAC53250.033.100912
ATCAACG53350.033.0388643
TCAACGC53550.032.9154664
CAACGCA54300.032.587395
AACGCAG55950.031.563866
CGCGTAT450.004560696430.75193239
ACGCAGA58850.029.9469597
AGAGTAC60850.028.68038211
CGCAGAG61450.028.679888
CAGAGTA63250.027.64643110
GCAGAGT64750.027.3243149
GAGTACG49900.026.0239412
AGTACGG50050.025.94594813
GTGGTAT10000.024.743731
TGGTATC10650.023.8739512
TCGTATG1803.274181E-1023.08494840
ACGAGAC2400.022.90847422
GTACGGG57550.022.32585314
Produced by FastQC (version 0.11.5)