FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311993
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42820.3263736925425669No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32200.24542813871720354No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTTTCAGGGAGA31320.2387207858578514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29380.22393412159973414No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28150.2145590715804124No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28120.2143304118238436No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG27820.21204381425815536No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27020.2059462207496534No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26850.20465048212909673No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24550.1871199007921536No Hit
GTCTCTGGTGGCTCCATCAGCACTGGTGGTTATCACTGGAGCTGGATCCG24540.18704368087329734No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24000.18292780525505853No Hit
CTCCTGGACCTCGCACAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTTTCAGGGAGAACTGGTT22920.1746960540185809No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22240.16951309953635424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21480.16372038570327738No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20650.1573941324382066No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20390.15541241454794347No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA19890.15160141860512977No Hit
GTCCAGGAGGGCACCGATGACTACGGTGACTGGGGGCCCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT19640.1496959206337229No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19440.1481715222565974No Hit
GTGTATTACTGTGCGAGGTCCAGGAGGGCACCGATGACTACGGTGACTGG19130.1458087047720529No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA19120.14573248485319662No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18670.1423025885046643No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAAACTGTCCCAGATCCTCTTCCA18260.13917757183155702No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGATAT18000.13719585394129388No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17940.13673853442815626No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17510.13346107791733644No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17500.13338485799848018No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17090.13025984132537294No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16790.1279732437596847No Hit
GTGGAAGAGGATCTGGGACAGTTTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTA16570.12629640554484667No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16290.12416224781687096No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16030.12218052992660783No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAAACTGTCCCAGATCCTCTTCCA15870.12096101122490745No Hit
ACATATTACTGTCAACACTTTGATAATCACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15530.11836953398379413No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15490.11806465430836903No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGTGGTTATCACTGGA15400.11737867503866255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15050.11471097787869296No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14990.11425365836555532No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14940.11387255877127393No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14690.11196706079986707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14620.11143352136787316No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14600.1112810815301606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14310.10907070388332864No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14110.10754630550620316No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14100.10747008558734689No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14090.1073938656684906No Hit
ATATTACTGTCAACACTTTGATAATCACCCGCTCACTTTCGGCGGAGGGA14050.10708898599306552No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14020.1068603262364967No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA13900.10594568721022139No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG13740.104726168508521No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13580.10350664980682062No Hit
CTCTTCCACTGAACCTTGATGGGACCCCTGTTTCCAAATTAGATGCATCGTAGATCAGGAGCTTAGGGACTTTCC13530.10312555021253925No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13500.10289689045597042No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13200.10061029289028219No Hit
GATCTACGATGCATCTAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGAGGATCTGGGACAGTTTTTA13140.10015297337714454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)