FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311993
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37310.28437651725275975No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTTTCAGGGAGA30280.23079391429679882No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29110.22187618379061474No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28630.21821762768551356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26330.20068704634857046No Hit
GTCTCTGGTGGCTCCATCAGCACTGGTGGTTATCACTGGAGCTGGATCCG25600.19512299227206242No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25460.19405591340807457No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25030.19077845689725478No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24630.1877296601430038No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24070.18346134468705244No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23690.18056498777051402No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA22610.17233323653403637No Hit
CTCCTGGACCTCGCACAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTTTCAGGGAGAACTGGTT22490.1714185975077611No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22100.1684460206723664No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21750.1657783235123968No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA21320.16250086700157698No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20880.15914719057190094No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20570.1567843730873564No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20060.15289715722568642No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18550.14138794947838898No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAAACTGTCCCAGATCCTCTTCCA18460.14070197020868253No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18410.14032087061440116No Hit
GTCCAGGAGGGCACCGATGACTACGGTGACTGGGGGCCCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT18200.1387202523184194No Hit
GTGTATTACTGTGCGAGGTCCAGGAGGGCACCGATGACTACGGTGACTGG18010.13727207386015017No Hit
GTGGAAGAGGATCTGGGACAGTTTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTA17960.1368909742658688No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17390.13254643889106116No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17190.13102204051393568No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGATAT16780.1278970238408284No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG16380.12484822708657745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16280.1240860278980147No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGTGGTTATCACTGGA16220.12362870838487705No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15980.12179943033232647No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA15810.1205036917117698No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15380.11722623520094999No Hit
ACATATTACTGTCAACACTTTGATAATCACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14940.11387255877127393No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14930.11379633885241766No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAAACTGTCCCAGATCCTCTTCCA14730.11227194047529215No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14690.11196706079986707No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14670.11181462096215451No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14530.11074754209816667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14510.11059510226045413No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14490.11044266242274157No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14450.11013778274731648No Hit
ATATTACTGTCAACACTTTGATAATCACCCGCTCACTTTCGGCGGAGGGA14000.10670788639878413No Hit
GTCCCTAAGCTCCTGATCTACGATGCATCTAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGAGGAT13810.10525970794051492No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13750.10480238842737728No Hit
CTCTTCCACTGAACCTTGATGGGACCCCTGTTTCCAAATTAGATGCATCGTAGATCAGGAGCTTAGGGACTTTCC13680.10426884899538336No Hit
GATCTACGATGCATCTAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGAGGATCTGGGACAGTTTTTA13620.1038115294822457No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13550.1032779900502518No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT13370.10190603151083887No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13350.10175359167312631No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13140.10015297337714454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3600.043.164911
GTATCAA22650.038.8770031
TCAACGC26300.033.1733444
TATCAAC26300.033.0699542
GTAGTTT2700.032.7851470
ATCAACG26750.032.6302153
TATTGAT10500.032.5175170
CAACGCA27600.031.7345665
AACGCAG27850.031.5735156
AGAGTAC29800.029.50746211
CAGAGTA30250.029.18250310
CGCAGAG30650.028.5766418
TGTTAGA7550.027.63629570
GCAGAGT32300.027.2236029
ACGCAGA32350.027.1815267
CCGGTAT700.001282539824.80959548
AGCCTAA850.001424617124.36580869
CGAAATT2001.8189894E-1224.31340448
CTATTGA10150.023.66964169
AGTACGG28350.023.47893713
Produced by FastQC (version 0.11.5)