FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812743
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCTTCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT20100.11088168593121034No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19830.10939223044855227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.038.979231
GTATCAA41750.037.7515221
TCAACGC50650.031.520454
ATCAACG50650.031.4506363
TATCAAC50400.031.4005372
GTGGTAT9350.030.2466321
CAACGCA52750.030.2002375
AACGCAG53100.030.0661166
ACGCAGA57000.027.9461547
CGCAGAG57550.027.7389878
AGAGTAC57600.027.23302711
GCAGAGT62200.025.7199759
TGGTATC10700.024.8120062
ATAAGCG700.001338019524.6305922
CAGAGTA70300.022.36353710
GCGGAAT800.002796213121.69843345
AGTACGG52350.021.60051713
GAGTACG52450.021.55933612
TCGTATG900.00562409119.23193740
TGTTAGA4859.094947E-1218.92841170
Produced by FastQC (version 0.11.5)