FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812743
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20270.11181949123510614No Hit
GTATAATAACTGGCCCTTCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT20090.1108265209133341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42200.038.9724731
GGTATCA18350.038.5185471
TCAACGC49600.032.7579734
TATCAAC50350.032.6487852
ATCAACG49800.032.6390153
AACGCAG51550.031.7209056
CAACGCA51450.031.7156495
GTGGTAT8700.030.1194061
ACGCAGA56300.029.0446287
CGCAGAG56800.028.729148
AGAGTAC58100.028.09019511
CGATTGT1355.608874E-527.98416970
TGGTATC9900.027.5004622
GCAGAGT62800.026.2035949
AGTACGG53750.024.02576413
GAGTACG54650.023.56448212
CAGAGTA70500.023.43995310
ACGAACT10900.020.8271855
TTATACG1004.308093E-420.71990835
ACACGAC10300.020.48059338
Produced by FastQC (version 0.11.5)