FastQCFastQC Report
Wed 8 May 2024
EGAF00005760426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382944
Total Bases28.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12530.327201888526782No Hit
GTGTAAGCTCCTGCCAATGGTCTGACTGGCCCGGCAGGTGATGGTGACTT12210.31884557533216346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11200.2924709618116487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10160.26531294392913846No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGGGT9390.24520556530458765No Hit
GTATTACTGTCATCAGAGTACTGATTTCCCGCTCACTTTCGGCGGAGGGA9070.2368492521099691No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8700.22718726497869138No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8650.22588159104203223No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT8150.2128248516754408No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7750.20237946018216765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7620.19898470794685383No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7460.19480655134954458No Hit
CCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTC7340.19167293390156265No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7280.19010612517757164No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6890.17992186847163033No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA6860.17913846410963483No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG6830.17835505974763935No Hit
GTCCTCCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAG6800.17757165538564385No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTTTTGGAGTTGGATCCG6670.17417690315033008No Hit
GTAATAAACGGCCGTGTCTGCGACAGTCACAGAGTTCAGGTTCAGGGAGA6580.17182669006434362No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6480.1692153421910253No Hit
GATTTCACCCTCACCATCAATGGCCTGGAAGCTGAAGATGCTGCAACGTA6330.1652983203810479No Hit
GAGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCA6330.1652983203810479No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6220.16242583772039776No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC6210.16216470293306592No Hit
CCCCTGAGGAGGACTGCGAAGCATACTTGATGAGGAGCTTTGGAGACTGA6080.15876995069775215No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6040.15772541154842484No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5930.15485292888777472No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5800.15145817665246092No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT5720.1493690983538063No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5640.14728002005515167No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA5610.14649661569315617No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5460.14257959388317873No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5460.14257959388317873No Hit
GAGGAGGACTGCGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGG5440.1420573243085151No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC5380.1404905155845241No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA5360.13996824600986046No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA5340.1394459764351968No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT5250.13709576334921034No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC5160.13474555026322385No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5140.1342232806885602No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5050.13187306760257375No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATT5000.13056739366591458No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4910.12821718057992815No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4900.1279560457925963No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTT4820.12586696749394166No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG4780.12482242834461435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC4710.12299448483329156No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC4610.12038313695997326No Hit
AGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCT4420.1154215760006685No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT4410.11516044121333668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4380.1143770368513412No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG4300.11228795855268656No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC4280.1117656889780229No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA4200.10967661067936828No Hit
GTGATAGATGTATCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGA4190.10941547589203643No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4090.10680412801871815No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA4060.10602072365672266No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTTTTGGA4050.10575958886939084No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGACTTT4020.10497618450739532No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG4020.10497618450739532No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGCC4010.1047150497200635No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT4000.10445391493273168No Hit
GCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAG4000.10445391493273168No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3990.10419278014539984No Hit
AGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGG3960.10340937578340437No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT3930.10262597142140888No Hit
TCGCAGTCCTCCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG3890.10158143227208156No Hit
CACCATCAATGGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATC3880.10132029748474974No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGT3860.10079802791008606No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3850.10053689312275423No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT3840.1002757583354224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)