FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382944
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11020.2877705356396758No Hit
GTGTAAGCTCCTGCCAATGGTCTGACTGGCCCGGCAGGTGATGGTGACTT9850.25721776552185177No Hit
GTATTACTGTCATCAGAGTACTGATTTCCCGCTCACTTTCGGCGGAGGGA8650.22588159104203223No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8060.21047463858945437No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7930.20707988635414054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7880.2057742124174814No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA7870.2055130776301496No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7800.20368513411882677No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT7180.18749477730425337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7090.1851445642182669No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6910.18044413804629397No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6810.17783279017297568No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6750.1762659814489847No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6670.17417690315033008No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTTTTGGAGTTGGATCCG6650.1736546335756664No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG6540.1707821509150163No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA6460.16869307261636166No Hit
CCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGCCTGGAAG6250.16320924208239324No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6210.16216470293306592No Hit
GATTTCACCCTCACCATCAATGGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTC6110.15955335505974763No Hit
GTAATAAACGGCCGTGTCTGCGACAGTCACAGAGTTCAGGTTCAGGGAGA6060.15824768112308849No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC6060.15824768112308849No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5810.15171931143979275No Hit
GTCCTCCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGC5680.14832455920447898No Hit
GAGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCA5540.14466867218183338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5490.14336299824517423No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5430.14179618952118325No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG5180.13526781983788755No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5160.13474555026322385No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5040.13161193281524192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4960.12952285451658727No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4940.12900058494192362No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4930.1287394501545918No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC4840.12638923706860533No Hit
GAGGAGGACTGCGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCTGCTGGTACCAGTGTAAGCTC4800.12534469791927802No Hit
CCCCTGAGGAGGACTGCGAAGCATACTTGATGAGGAGCTTTGGAGACTGA4800.12534469791927802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4790.12508356313194618No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4700.12273335004595973No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGACTTT4680.12221108047129606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4610.12038313695997326No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4520.11803292387398678No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4520.11803292387398678No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4480.11698838472465947No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA4480.11698838472465947No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT4450.116204980362664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4360.11385476727667754No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT4340.11333249770201387No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG4290.11202682376535472No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4180.1091543411047046No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTT4160.10863207153004094No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTTTTGGA4150.10837093674270912No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4070.1062818584440545No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4040.105498454082059No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT4020.10497618450739532No Hit
GAGTACTGATTTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT4010.1047150497200635No Hit
CTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTC3980.10393164535806801No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT3950.10314824099607253No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG3940.1028871062087407No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3930.10262597142140888No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGCC3910.10210370184674522No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC3890.10158143227208156No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC3890.10158143227208156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGT401.07302185E-861.189158
GTCGTAA353.1394666E-759.52211448
GGTCGTA407.930703E-752.04759247
AGGTCGT407.959552E-752.02021846
CGTAAAC451.760407E-646.36211850
TCGTAAA451.7700222E-646.32547449
TATGGAC502.3427274E-644.4759768
GTATCAA9700.044.445451
TGGTATC1100.043.8729932
GGTATCA3350.043.240781
AACCCTA503.5627945E-641.8472854
GTGGTAT1250.041.3876041
CGTTAAA350.001354756639.38071417
TATCAAC10900.039.2154432
TTGATAT556.4561336E-638.37789564
AAACCCT556.9190555E-637.99275253
TAAACCC556.9504767E-637.9676852
ATCAACG12050.035.1638373
ACCCTAT601.2428231E-534.8819655
GTAAACC601.2643817E-534.79452551
Produced by FastQC (version 0.11.5)