FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690171
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.041.701451
GTATCAA19700.037.4302141
TCGTATG751.6214071E-632.31686440
GTGGTAT3150.031.5745771
TCAACGC23550.031.1586444
ATCAACG23950.030.6382483
CAACGCA24450.030.2921835
AACGCAG24750.029.7864636
TATCAAC24950.029.6872752
ACGCAGA25750.028.7628697
CGCAGAG27150.027.2797018
AGAGTAC27250.027.0537611
CGTATGC906.6673856E-626.95240841
CAGAGTA28400.026.0790110
TGGTATC3750.025.6043592
GCAGAGT28850.025.553389
TCCGAAT700.001344319124.60807434
TTAGACT851.50377E-424.2040484
GAGTACG24300.023.70602212
AGTACGG24450.023.42034513
Produced by FastQC (version 0.11.5)