FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690171
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10960.15880122462404245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.037.2534681
GGTATCA9900.035.0267751
ATCAACG25750.031.1342813
AACGCAG26100.030.7056476
TCAACGC25900.030.6782884
GTGGTAT4600.030.601311
CAACGCA26400.030.3567185
TATCAAC26950.029.6552312
ACGCAGA27650.029.108227
AGAGTAC28150.028.59534311
ACCTATA605.6067656E-428.5839922
CGCAGAG28400.028.0983268
CAGAGTA29100.027.3047310
GCAGAGT30150.026.921799
TGGTATC5100.025.5574512
GAGTACG26150.025.2826712
AGTACGG26350.024.96076613
GTCTAGC902.1960684E-422.8887841
TACGGGG11250.021.6209415
CGAACTA1756.524715E-921.58712429
Produced by FastQC (version 0.11.5)