FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462695
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36860.25200058795579394No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33390.2282772553403136No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT30730.21009164590020477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25700.17570306865067564No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT23270.15908989912456117No Hit
GTATAAAAACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23150.1582694956911728No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22790.1558082853910077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22160.15150116736571878No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21880.14958689268781258No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAACCTGCAGTCTGAAGATT20880.14275019740957615No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20790.1421348948345349No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20610.14090428968445232No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGATAACACTCTGA20570.14063082187332288No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20260.13851144633706958No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC19570.13379412659508647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19560.1337257596423041No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17480.11950543346357237No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTAACAGACCTCAGTTTCAGGGAGA17420.11909523174687818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16970.11601871887167181No Hit
CTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT16940.11581361801332472No Hit
GGATACCAGTGGCCCTGGTGGACGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG16890.1154717832494129No Hit
TCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT16720.11430954505211272No Hit
GAGTGAACTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCA16450.11246363732698887No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15860.1084299871128294No Hit
GTCTCCCGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG15570.10644734548214084No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15570.10644734548214084No Hit
GTTTTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGTTGCTGATGCTGAGAGTGAACT15480.10583204290709958No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15200.10391776822919338No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15130.10343919955971682No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15070.10302899784302263No Hit
GTCATAGATGTACCCAATCGACTCCAGGCCCTTCCCTGGGCGCTGGCGGATCCAGCTCCAGTAGTAACCACCA14970.102345328315199No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC14930.10207186050406954No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14930.10207186050406954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14920.10200349355128718No Hit
GAGTTCACTCTCAGCATCAGCAACCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAAAAACTGG14760.10090962230676935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.039.1441081
GTATCAA23000.038.955011
TATCAAC26650.033.3565332
TCAACGC27950.031.803984
ATCAACG28100.031.6342073
CAACGCA28650.031.1471755
AACGCAG29650.029.9804766
TGTTAGA7450.029.33703670
AGAGTAC31900.027.75596211
CTAGTAC756.2205334E-527.5634483
CGCAGAG32700.027.0769188
ACGCAGA34000.026.1429527
GCAGAGT34800.025.7399659
GTTTCGT1101.0028343E-625.49610961
CACTGAT9400.025.30273470
ATAGAAC1101.1665325E-625.057683
CAGAGTA38100.023.42009410
GTCTAGT750.002006703522.9734671
AGTACGG33400.022.79605313
TATTCTA2002.7025795E-522.49891970
Produced by FastQC (version 0.11.5)