FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437531
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42070.2926545584060448No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38710.26928114941521264No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCAATGACGTTCGGCCAAGGGA38270.26622034585688936No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACTTCAGGTTCAGGGAGA37970.26413343433985076No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32610.22684728190209463No Hit
GTATGGTAGCTCACCAATGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26760.18615250731984215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26430.18385690465109972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25900.18017002763766485No Hit
GTGTATTACTGTGCGAGACCGACGAGCGACGATCTAGTCGATCTAGTGTA25090.17453536654166066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24240.16862245057671799No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT23700.16486600984604854No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23410.16284866204624457No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGTAGCTACTTAGCCTGGTATC22950.15964873105345206No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22840.15888353016387124No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT22300.1551270894332018No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22200.1544314522608556No Hit
GTCTCTGGTGACTCCATCAGCAGTCGTAGGTACTACTGGGGCTGGATCCG22120.15387494252297865No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22030.15324886906786706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21750.1513010849852977No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21580.15011850179230918No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20510.14267518404820487No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20430.1421186743103279No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC20020.1392665619037085No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20010.13919699818647388No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19980.13898830703477003No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19700.13704052295220068No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19620.13648401321432374No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19270.13404928311111206No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCAATGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19070.13265800876641964No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19030.13237975389748116No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18760.13050153353214644No Hit
GTGTTAGTAGTAGCTACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT17770.1236147255259191No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17530.12194519631228823No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17520.1218756325950536No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC17480.12159737772611512No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGACTTCAGGTTCAGGGA17190.11958002992631116No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16680.11603228034734556No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTCGTAGGTACTACTGGG16630.11568446176117245No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTAGCTACTACTAACACTCTGA16130.11220627589944147No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15700.10921503605835284No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15340.10671074223790653No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15320.1065716148034373No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14920.1037890661140525No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14420.10031088025232152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAA12100.045.05908670
GTATCAA26200.038.4394451
TATCAAC29500.033.6612362
ATCAACG30850.032.1882133
TCAACGC31050.031.9808854
CAACGCA31850.031.285855
AACGCAG32550.030.6130396
TGTTAGA6850.029.61615870
AGAGTAC34900.028.35213911
CAACGGA12650.028.32997369
CGCAGAG35400.028.2438628
AACTGTG21250.028.04389270
TCGTATG756.0560367E-527.67206840
GCAGAGT36700.027.24349
ACGCAGA37600.026.6829917
GAGCAAA13700.026.3768970
CAGAGTA38650.025.60128410
TCAACGG13200.025.31662868
GTCAACG13650.024.12852767
CTAGTCG10050.024.05817634
Produced by FastQC (version 0.11.5)