FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321932
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC23630.1017686995140254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.043.4706461
GTATCAA54450.041.939441
TCAACGC66600.034.277254
CAACGCA67300.033.8695645
ATCAACG67950.033.545573
AACGCAG68550.033.3524176
TATCAAC71150.032.1343272
ACGCAGA74400.030.7722517
AGAGTAC74800.029.82459811
CAGAGTA75350.029.7896610
CGCAGAG78150.029.2075488
GCAGAGT79550.028.8660169
TGGTATC8200.026.4546952
GTGGTAT8300.026.1438451
AGTACGG68400.025.41771513
TATACCG951.0674972E-525.3712065
GAGTACG69500.025.06495512
ACGGTAT1551.8233526E-423.52821570
GTACGGG80700.021.54363814
ATAACTC1354.8684924E-621.25919267
Produced by FastQC (version 0.11.5)