FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400791
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA59620.42561666943890986No Hit
GTCTATTACTGTGCGAGTGGGTTCGGCGTAGTTTATCAGTGGGAGCTACT38700.2762724774787959No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26750.19096353417461992No Hit
CACCTGCACTGTCTCTCGTACTTCCATCAGCAGAAGTTCTTACTACTGGG23940.1709034395566505No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23200.1656207100131283No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22610.16140880402572547No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21030.15012946256793483No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20570.1468456036625021No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20350.1452750624468604No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20350.1452750624468604No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16480.11764781469898078No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15440.11022343804321987No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15380.10979510862077213No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15220.10865289682757814No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14540.10379849670650368No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14510.1035843319952798No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14460.10322739080990669No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14410.10287044962453357No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14280.10194240254256345No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14220.1015140731201157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG851.8189894E-1244.76860440
GTATCAA29650.037.3494641
GTGGTAT4500.036.7987251
CGTATGC952.1282176E-1036.43683241
CTACGGA13600.035.66517369
CTCGTAT1003.7107384E-1034.58277539
AACGAAT400.002601519534.4864917
TATCAAC32550.034.0145762
ATCAACG34100.032.6695863
TCAACGC34700.032.204094
CAACGCA35150.031.9880525
AACGCAG36350.030.9320476
GGTATCA18850.030.7469431
GTGTCTA14100.030.53810365
ACGTGTC14300.029.99812563
TTGGACG14450.029.56942459
TCTACGG15300.029.45573468
GACGTGT14550.029.45160962
AGAGTAC39150.028.54054311
CAGAGTA39850.028.03920210
Produced by FastQC (version 0.11.5)