FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400791
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA55680.3974897040315079No Hit
GTCTATTACTGTGCGAGTGGGTTCGGCGTAGTTTATCAGTGGGAGCTACT38300.27341694799581095No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27380.19546099311032122No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25070.17897031034608302No Hit
CACCTGCACTGTCTCTCGTACTTCCATCAGCAGAAGTTCTTACTACTGGG24480.1747584043586802No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23180.16547793353897905No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19010.13570903867886072No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18870.134709603359816No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17730.12657134433330883No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17270.12328748542787611No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16300.11636282643163755No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15140.10808179093098115No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14500.10351294375820519No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14470.1032987790469813No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14380.10265628491330969No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14200.10137129664596646No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14130.10087157898644408No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14130.10087157898644408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT306.0934294E-446.33000648
GTATCAA29350.038.9333651
GTGGTAT4750.037.7930371
AATAGAC12600.036.91313
CTACGGA15600.035.4669669
CCGTGTC13250.034.8410112
TAATAGA13600.034.7415242
TATCAAC33350.034.0225832
ATAGACA13850.033.8243064
GCGGTCA13750.033.336623
GTCTGCG14350.032.41497416
GTAATAG14500.032.379711
TCAACGC35000.032.379314
ATCAACG35050.032.3388943
TAGACAG15150.032.286095
CGGCGGT14550.031.97625721
AGCCGTG14450.031.94421410
CAGCCGT14500.031.8329269
CAACGCA35750.031.5073185
GCCGTGT14850.031.31685311
Produced by FastQC (version 0.11.5)