FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882846
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19900.22540737569179675No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19650.22257562474089476No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC19140.21679885280105476No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15820.1791932001730766No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA15430.17477566868966954No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA15250.17273680800502011No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14840.1680927364455409No Hit
GTATGATAATCTCCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14830.16797946640750483No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA14550.16480790534249462No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13570.1537074416149589No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA13470.1525747412345981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13060.14793066967511886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12600.14272024792545926No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA12250.1387557965941965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12180.13796290632794395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12150.1376230962138357No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11730.13286575461632041No Hit
ATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC11490.1301472737034545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11300.12799514298076903No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11270.12765533286666078No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11160.12640936244826392No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10710.12131221073664036No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA10250.11610178898698074No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9970.11293022792197054No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG9870.11179752754160975No Hit
GATCTACGATGCGTCCCATTTGCAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA9760.11055155712321287No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT9750.11043828708517679No Hit
ACCTTGATGGGACCCCTGTTTGCAAATGGGACGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG9550.10817288632445524No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9240.1046615151453368No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9230.1045482451073007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9150.10364208480301208No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC9070.10273592449872346No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8940.10126341400425443No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG8910.10092360389014618No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT8880.10058379377603795No Hit
ATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGC8840.10013071362389363No Hit
GTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCA8830.10001744358585755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG651.1114025E-842.5748640
GTATCAA19950.041.6150441
GTGGTAT2350.039.579671
CGATTGT650.001713737.54355670
TATCAAC23100.036.083262
ATCAACG24150.034.7996673
GTATAGC400.002617101934.4431153
TCAACGC24500.034.3025324
CAACGCA24800.033.7486955
AACGCAG25150.033.2771456
CTCGTAT751.6400973E-632.27124439
TGTTAGA4950.032.04475470
CGCAGAG27850.029.9188568
ACGCAGA28200.029.7917147
AGAGTAC28000.029.63392811
CAGAGTA28200.029.54584710
GCAGAGT28850.029.1188479
GTATAGA854.477899E-628.369741
GTAGTTT2853.6379788E-1227.82833970
AGTACGG24600.027.71151513
Produced by FastQC (version 0.11.5)