FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685424
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53100.039.3848881
GGTATCA22550.036.3963851
ACGCGTA1003.965397E-1034.3680527
TCAACGC64150.033.0795824
ATCAACG64900.032.6973043
CAACGCA66250.032.1865125
TATCAAC66650.031.581192
ACGTTAG953.153729E-431.53585470
AACGCAG68800.031.0434636
GTGGTAT9250.030.4424041
ACGCAGA74850.028.5325767
AGAGTAC73650.028.34479111
CGCAGAG75850.028.0658728
CAGAGTA75000.027.92614710
TGGTATC10050.027.3333032
GCAGAGT79700.026.7101159
AGTACGG59200.024.76558713
GAGTACG59450.024.6614412
TCGTATG1202.384062E-623.08201840
ACGGGAT6100.023.08064816
Produced by FastQC (version 0.11.5)