FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685424
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17310.10270412667672943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58650.039.697471
GGTATCA24400.037.1548271
GTGGTAT10350.033.8423841
ATCAACG69300.033.502433
TCAACGC69500.033.348764
TATCAAC69350.033.3101232
CAACGCA70500.032.8757255
AACGCAG71850.032.3057376
TGGTATC11100.030.9232182
ACGCAGA77500.029.9947857
CGCAGAG78400.029.694198
AGAGTAC77950.029.07561511
CAGAGTA79800.028.57257710
GCAGAGT84850.027.3965349
AGTACGG65000.025.4274613
GAGTACG65550.025.37029512
GTACGGG75650.021.66647514
TACGGGG30800.021.26623715
GAGTACT29100.020.73844112
AGTACTT29750.020.40119613
Produced by FastQC (version 0.11.5)