FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548032
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG200.007030124352.34813751
GTATCAA13650.039.8537331
ACGGGTA557.499475E-637.553816
GGTATCA6100.037.252781
TCGTATG1151.8189894E-1236.13201540
CGTATGC1250.036.03803341
TATACAC1450.033.237273
CTCGTAT1154.5474735E-1133.10278339
TCTCGTA1154.5474735E-1133.07547838
TATCAAC16900.031.9799862
GAACGAA450.004254442631.18844259
CAACGCA17600.030.512465
ATCAACG17600.030.512463
TCAACGC18050.029.7517624
AACGCAG18250.029.4257166
ACGAGAC1601.8189894E-1227.97996722
TACACAT1900.027.1770925
TATGCCG1554.9112714E-1126.85938843
CAGAGTA19950.026.7457110
TTATACA1555.4569682E-1126.6510832
Produced by FastQC (version 0.11.5)