FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548032
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9340.17042800420413406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3100.041.08761
GTATCAA14550.039.5115661
TGGTATC3150.037.1195562
GTTAGCG400.00232801335.2724764
TATCAAC16550.034.7018742
ATCAACG17000.033.3665163
TCAACGC17050.033.2656334
CAACGCA17450.032.5030985
AACGCAG17700.032.2382166
ACGCAGA18800.030.1690987
CAGAGTA19250.029.28527810
CGCAGAG19750.028.7179248
AGAGTAC19850.028.57585711
TGTGCGA500.007044555628.13105662
GCAGAGT20600.027.5329659
AGTACGG16900.027.06198713
GAGTACG17000.026.8978912
GGTATCA6400.026.8943331
TACGGGA5500.026.25919215
GTGTTAC1101.1730426E-625.0361791
Produced by FastQC (version 0.11.5)