FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753796
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21600.040.86211
GGTATCA9000.039.6855241
ATCAACG25950.033.8732953
TATCAAC26450.033.3627852
TCAACGC26450.033.232974
CAACGCA26650.032.9835665
AACGCAG27900.031.628886
TCGTATG1101.024091E-931.45698740
ACGCAGA29100.030.4405697
GTGGTAT3000.029.7641431
CGCAGAG30050.029.4782188
AGAGTAC30000.029.1840111
CAGAGTA30650.028.565110
CGTATGC1253.963578E-927.71735641
GCAGAGT32200.027.5099549
CGATTGG1005.226502E-727.46912416
AAACGTA658.890968E-426.41612425
ACGAGAC1306.6265784E-926.4126222
CTTATAC1457.403287E-1026.0534931
CTCGTAT1359.23319E-925.60252439
Produced by FastQC (version 0.11.5)