FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753796
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13000.17246045349139555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.036.938091
GGTATCA9450.036.7099341
GTGGTAT3650.036.6999741
TGGTATC4200.031.8707262
ATCAACG24200.031.037613
CTAACGA450.00452520530.7991741
TCAACGC24600.030.6642134
TATCAAC24750.030.5087282
AACGCAG24650.030.4629146
CAACGCA24900.030.157065
ACGCAGA26150.028.7174227
CAGAGTA26800.027.3811710
AGAGTAC26850.027.20608311
CGCAGAG27950.026.7453168
GCAGAGT29300.025.5130239
GTATTAT700.001368304724.5338651
AGTACGG25800.022.73341813
GAGTACG26200.022.38337112
TACGGGG11650.021.19940615
GCTTAAC1305.1105817E-621.1368681
Produced by FastQC (version 0.11.5)