FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769085
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC200.00723521451.97090541
ATTCGAC200.00736121151.74448820
GTATCAA15800.041.7121051
CAACGCA17600.037.2427755
ATCAACG17750.036.9280473
GGTATCA6400.035.583591
TATCAAC18550.035.521442
TCAACGC18500.035.430964
AACGCAG18750.035.144836
AGAGTAC19500.032.72515511
ACGCAGA20200.032.6156927
CGCAGAG20950.031.1187678
CAGAGTA20400.031.1123110
TCTTACG450.00447172830.87419747
GCTATAC450.00462268630.6653963
GTGGTAT2450.029.5759721
GCAGAGT22600.028.999459
TACGCTT500.007345250327.8926954
AGTACGG18550.026.77533713
GAGTACG18600.026.70509712
Produced by FastQC (version 0.11.5)