FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808416
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT20860.25803546688833473No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19820.24517080315085302No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGA17600.21770969401892093No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15670.19383584689070973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13250.1639007639631081No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12750.1577158294739342No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12370.15301527926216205No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11430.14138760242251516No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCCCACTTTCAGGGAGA11290.13965582076554645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11270.1394084233859795No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11040.1365633535209595No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10530.13025472034200214No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10520.13013102165221865No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10050.12431718323239521No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAGCTACTTAGCCTGGTTCCAACAGAAACCTGGCCGGGCTCCCAG9430.11664786446581958No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9300.11503978149863436No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9090.11244210901318133No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT8790.108731148319677No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAA8700.10761786011162569No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8680.10737046273205873No Hit
GTACCTCACCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGA8660.10712306535249179No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAA8560.105886078454657No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8500.10514388631595614No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8350.10328840596920397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8160.10093813086331789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)