FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808416
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT19870.24578929659977042No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19680.24343902149388436No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGA17370.21486462415390095No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15310.18938269405850452No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12520.15487075960891422No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12240.15140719629497684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11220.1387899299370621No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10950.1354500653129082No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10670.13198650199897083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10610.13124430986026994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10320.1276570478565491No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.12679115702806476No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10220.12642006095871433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10110.12505937537109607No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAGCTACTTAGCCTGGTTCCAACAGAAACCTGGCCGGGCTCCCAG9880.12221430550607608No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAA9510.1176374539840874No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9430.11664786446581958No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8960.11083402604599613No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8740.1081126548707596No Hit
GTACCTCACCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGA8520.1053912836955231No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTC8460.10464909155682223No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8450.10452539286703874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTAG250.00843286949.9948769
GTATCAA20500.039.446931
GGTATCA8350.038.73831
TATCAAC23350.034.457092
AACGCAG24900.032.846226
ATCAACG24750.032.7696233
TCAACGC24950.032.6426624
CAACGCA25200.032.4551965
CGCGTAT450.004707562330.55173112
AGAGTAC27000.029.91338711
CGCAGAG27600.029.5084938
GCAGAGT27700.029.2779059
ACGCAGA28300.028.9000327
CAGAGTA29200.027.6562210
TACGGGT1800.026.74600415
GAGTACG23750.026.33881212
TACCACG3009.640644E-1125.44392270
TGGTATC5350.025.064582
GTGGTAT5700.024.7518791
ATATACG700.001363982424.5474623
Produced by FastQC (version 0.11.5)