FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612529
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGACCC24750.40406250153053974No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGGCCAGGGAGG24340.3973689408991248No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGG23740.38757348631656624No Hit
GTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCT23060.3764719711229999No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG21350.34855492556270806No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCAC18000.2938636374767562No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCATG16310.2662731070692163No Hit
GTATATGAATAGTCTGCGGCCCTCAGGGGTCCCTGACCGATTCTTTGGTTCCAAGTCTGGCACCTCAGCCTCC15040.2455393948694674No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC14890.2430905312238278No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14040.22921363723186983No Hit
ATTCATATACATGACAAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACC13920.22725454631535813No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13740.22431590994059056No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13650.22284659175320679No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13530.22088750083669506No Hit
GAGTGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGAAATT12690.2071738644211131No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12430.2029291674353378No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12420.20276590985896176No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12210.19933750075506626No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11130.1817056825064609No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10930.17844053097894141No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10910.17811401582618946No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10900.1779507582498135No Hit
CCTCAGGGGTCCCTGACCGATTCTTTGGTTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCGGT10620.17337954611128617No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10320.16848181882000687No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10260.16750227336175105No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9820.1603189400012081No Hit
GTGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGAAATTAT9730.1588496218138243No Hit
GTATTACTGTGCGAGGTTTAAAGATTACACCGCCGGTATGGACGTCTGGGGCCAGGGGACCACGGTCACCGTCTC9440.15411515209892102No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9400.15346212179341712No Hit
CCCCTGAGGGCCGCAGACTATTCATATACATGACAAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTAGA9360.15280909148791322No Hit
GCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG9360.15280909148791322No Hit
CAGTAATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTA9330.1523193187587853No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCAC9180.14987045511314567No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8760.14301363690535468No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8760.14301363690535468No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8680.14170757629434688No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8640.14105454598884296No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8540.13942197022508324No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8300.13550378839205982No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8300.13550378839205982No Hit
GTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGACCCCCGGGCAGAGTGTCACCATCTCTTGTTCTGGAAGCAG8040.13125909140628442No Hit
TCAGTGGACTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG8020.1309325762535325No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG8000.13060606110078052No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7910.12913674291339675No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC7890.1288102277606448No Hit
ACACCGGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC7840.12799393987876492No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT7780.12701439442050907No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7740.12636136411500518No Hit
GTAATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGGCTGTGGTTGTGGCGGTGTTCCTGGT7640.12472878835124541No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATG7550.12325947016386164No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7490.12227992470560578No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7440.12146363682372589No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7390.12064734894184602No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7270.11868825802533432No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT7210.11770871256707846No Hit
CTATTCATATACATGACAAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA7190.1173821974143265No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAG7190.1173821974143265No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7180.11721893983795054No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7030.1147700761923109No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG7010.11444356103955894No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGAC6970.11379053073405504No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6940.11330075800492712No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6890.11248447012304724No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6870.11215795497029528No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGAAATTATGTCTACTGGTACCAGCAACTCCCAGGAACGGCCCC6870.11215795497029528No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6850.11183143981754334No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC6780.1106886367829115No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGA6760.11036212163015954No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6700.1093825761719037No Hit
CTGCTGACTCAGCCACCGTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC6660.1087295458663998No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6560.10709697010264004No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC6490.1059541670680082No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6480.10579090949163222No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6290.1026890155404887No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6210.10138295492948088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)