FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612529
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGACCC26340.4300204561743199No Hit
GTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCT21460.3503507589028438No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGGCCAGGGAGG21100.3444734861533087No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGG20440.33369848611249425No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG20100.3281477285157111No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCAC16550.2701912889022397No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCATG15810.2581102282504176No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15070.24602916759859533No Hit
GTATATGAATAGTCTGCGGCCCTCAGGGGTCCCTGACCGATTCTTTGGTTCCAAGTCTGGCACCTCAGCCTCC14780.24129469788369204No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13440.2194181826493113No Hit
ATTCATATACATGACAAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACC13250.21631628869816774No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13220.21582651596903984No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12840.20962272806675275No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC12750.20815340987936898No Hit
GAGTGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGAAATT12710.20750037957386508No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12670.20684734926836118No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12350.20162310682432993No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11340.1851340916103564No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11210.18301174311746873No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11210.18301174311746873No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10310.16831856124363093No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10160.16586969759799128No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10110.1650534097161114No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9830.16048219757758408No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9670.1578700763555685No Hit
CCTCAGGGGTCCCTGACCGATTCTTTGGTTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCGGT9510.15525795513355287No Hit
CAGTAATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTA9360.15280909148791322No Hit
CTGCTGACTCAGCCACCGTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC9260.15117651572415347No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9050.14774810662025797No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCAC8980.14660530358562615No Hit
GTGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGAAATTAT8950.14611553085649823No Hit
GTATTACTGTGCGAGGTTTAAAGATTACACCGCCGGTATGGACGTCTGGGGCCAGGGGACCACGGTCACCGTCTC8920.14562575812737028No Hit
CCCCTGAGGGCCGCAGACTATTCATATACATGACAAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTAGA8840.14431969751636248No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8810.14382992478723455No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8690.14187083387072286No Hit
GCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG8590.1402382581069631No Hit
GTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGACCCCCGGGCAGAGTGTCACCATCTCTTGTTCTGGAAGCAG8560.13974848537783516No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8440.13778939446132346No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8280.13517727323930784No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8240.13452424293380394No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC8180.1335446974755481No Hit
ACACCGGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC7880.12864697018426882No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7800.12734090957326102No Hit
GTAATACAGGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGGCTGTGGTTGTGGCGGTGTTCCTGGT7700.12570833380950128No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7650.12489204592762138No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7510.12260643985835772No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7510.12260643985835772No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7440.12146363682372589No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7390.12064734894184602No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATG7380.12048409136547004No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7290.11901477317808626No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT7200.11754545499070249No Hit
CTATTCATATACATGACAAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA7130.11640265195607066No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7130.11640265195607066No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7010.11444356103955894No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG7000.11428030346318296No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGAC6980.11395378831043101No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6970.11379053073405504No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC6960.11362727315767907No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6830.11150492466479138No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6740.11003560647740761No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG6630.10823977313727186No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6590.10758674283176796No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6590.10758674283176796No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6450.1053011367625043No Hit
TCAGTGGACTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG6450.1053011367625043No Hit
GTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC6440.10513787918612832No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6410.1046481064570004No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6410.1046481064570004No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6380.10415833372787248No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGAAATTATGTCTACTGGTACCAGCAACTCCCAGGAACGGCCCC6310.10301553069324064No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC6290.1026890155404887No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6230.10170947008223284No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6220.10154621250585687No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC6170.10072992462397698No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCGTCAG6140.10024015189484906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG150.002281418369.5546655
ATAAGAC200.007327211651.803843
TAACCGG200.007329578551.7996114
GTGGTAT1250.047.0225831
GGTATCA4100.043.008461
GTATCAA10850.042.0641141
TCGCATG350.00123365740.1416866
GTACGAC350.001342609939.45670322
TGGTATC1600.036.703382
TATCAAC12800.035.623872
CCCTATA400.002579088634.5443572
CCTATAC400.0025821934.5358963
GGTACGA400.002585294734.52743521
TCAACGC14100.033.0527844
ATCAACG14050.032.938153
TTCGGGG4650.031.97115970
CAACGCA14700.031.706285
AACGCAG15050.031.1983266
ACGCAGA15900.029.7476257
GATGTTG7250.029.42113770
Produced by FastQC (version 0.11.5)