FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370705
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52070.3798775082895299No Hit
GAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCAT44690.3260366016028248No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39580.2887565158075589No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38140.2782509730394213No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC35530.259209676772172No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34480.251549385170405No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32710.23863632218456926No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32570.2376149499710003No Hit
GTCCTGATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTT31900.23272695437749186No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTG31440.22937101710433683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31150.2272553175190869No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30920.22557734888250935No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT30580.2230968735066991No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28610.20872470735862203No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA26420.19274752773207948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25730.1877136218223469No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25360.18501428097220043No Hit
GTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCA24280.17713512389609726No Hit
GGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT24140.17611375168252835No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23980.1749464691527353No Hit
GATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA23840.17392509693916633No Hit
GGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCA23250.16962074261055443No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23180.16911005650376995No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22700.16560820891439076No Hit
ACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGA22360.1631277335385805No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22170.16174158553445125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22110.16130385458577884No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC21870.15955293079108926No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTC21310.15546744193681353No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAA20940.15276810108666708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20900.15247628045421882No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAACTATTTAAATTGGTATCAGCAGAAA20670.1507983118176413No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTGAACGGGTTA20340.14839079159994312No Hit
ATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA20190.1472964642282621No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20170.14715055391203796No Hit
GTTTAGTCCCCTGGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAG19630.14321097537398636No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19360.1412411861049606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18950.13825002462236588No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA18860.13759342819935727No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18770.13693683177634866No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18530.13518590798165908No Hit
ACTTACTACTGTCAACAGAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAA18400.1342374909262022No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18110.13212179134095228No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18020.1314651949179437No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17910.13066268817871096No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17560.12810925764478864No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17400.12694197511499558No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC17300.1262124235338749No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17210.1255558271108663No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAAC17110.12482627552974565No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT16820.12271057594449572No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16650.1214703382565906No Hit
GGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16620.12125147278225439No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16340.11920872835511653No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16330.11913577319700445No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16270.11869804224833207No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16110.11753075971853899No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16090.11738484940231486No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCC15580.11366413633859948No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15470.11286162959936676No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15260.11132957127901336No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14990.10935978200998756No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG14770.10775476853152209No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA14630.10673339631795317No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14520.10593088957872043No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA14470.10556611378816011No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT14460.10549315863004805No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14340.10461769673270326No Hit
CCCCTAAGGTCCTGATCTATGCTACATCCAATTTACAAAGTGGGGTCCCA13960.10184540072444473No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13900.10140766977577231No Hit
ACCTTGATGGGACCCCACTTTGTAAATTGGATGTAGCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG13840.10096993882709993No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13790.10060516303653959No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13780.10053220787842752No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13750.10031334240409133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3100.045.5798341
GTATCAA25850.038.9289671
ATAGCTA700.002278564335.42909670
GCGACAA400.00261261734.45651216
TATCAAC30150.033.3708232
AATTGAT12000.032.55048470
TCAACGC31400.032.0423664
ATCAACG31550.031.7808113
CAACGCA32100.031.450965
AACGCAG32550.031.120886
TGGATCG10950.030.57579470
CGCAGAG34600.029.2759428
AGAGTAC34900.028.629411
TTGTATA756.21754E-527.5652122
ACGCAGA37400.026.9920397
GCAGAGT37300.026.9720349
AATAGCG2000.026.32838262
TAATAGC2150.026.10294561
TGTTAGA9900.025.67714970
AGTACGG31450.025.63509413
Produced by FastQC (version 0.11.5)