FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65144
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1750.26863563797126366No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1580.24253960456834092No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG1490.2287240574726759No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1370.21030332801178928No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT1320.20262802406975314No Hit
GTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGT1250.19188259855090262No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC1100.1688566867247943No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT1090.16732162593638708No Hit
GATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA1040.15964632199435097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG1010.15504113962912933No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA950.14583077489868598No Hit
GTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA930.14276065332187154No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG930.14276065332187154No Hit
GAGTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT920.1412255925334643No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAA910.1396905317450571No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC870.13355028859142823No Hit
GTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGA870.13355028859142823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC860.132015227803021No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT820.12587498464939212No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTCCGCCGAAAGTG780.11973474149576324No Hit
GGAGGGGACTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGT770.11819968070735601No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG770.11819968070735601No Hit
ACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCACTTTCGGCGG770.11819968070735601No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC760.11666461991894879No Hit
GACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGTCCC750.11512955913054157No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT740.11359449834213435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG730.11205943755372713No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT730.11205943755372713No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATT700.10745425518850547No Hit
GTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAACCAGGTAAAGCCCCT680.10438413361169101No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG670.10284907282328379No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGA670.10284907282328379No Hit
CCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATT670.10284907282328379No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG660.10131401203487657No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC660.10131401203487657No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT660.10131401203487657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)