FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65144
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3040.46665847967579516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1930.29626673216259364No Hit
GTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGT1160.17806705145523763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1120.17192680830160875No Hit
GTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGA1120.17192680830160875No Hit
GAGTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT1100.1688566867247943No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG1100.1688566867247943No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT1070.16425150435957264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1060.1627164435711654No Hit
GATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA1060.1627164435711654No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1050.1611813827827582No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1050.1611813827827582No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTCCGCCGAAAGTG1040.15964632199435097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT1020.15657620041753653No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAA1000.1535060788407221No Hit
ACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCACTTTCGGCGG980.15043595726390765No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC970.14890089647550045No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT910.1396905317450571No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC900.13815547095664987No Hit
CCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATT850.1304801670146138No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT840.12894510622620656No Hit
GGAGGGGACTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGT790.12126980228417046No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT770.11819968070735601No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG760.11666461991894879No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT720.11052437676531991No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT710.10898931597691269No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT710.10898931597691269No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG700.10745425518850547No Hit
GTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA680.10438413361169101No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA680.10438413361169101No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG680.10438413361169101No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG670.10284907282328379No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA660.10131401203487657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTGT200.00778007150.97228622
GTAGCCT200.00782758650.893934
AGCCTAG801.5702553E-646.95957670
ACGGGGA651.2863893E-841.7591216
GTATCAA2450.037.5646971
TATCAAC2700.035.24012
ATCAACG2650.034.6226843
CGGGGAA400.00279819933.92928317
CAACGCA2850.032.143535
TCAACGC2850.032.143534
GAGCCTA802.2262375E-531.95608169
TACGGGG1400.031.50576415
ATGGGAC652.4846859E-531.46444929
ACTGCCA652.5646135E-531.3193412
CACTGCC652.5646135E-531.3193411
TACGGGA553.5548443E-430.84480315
AACGCAG3000.030.5363566
AGTACGG2250.030.15936513
ACTTCGC853.1116433E-629.65108961
GCGTAGA850.005548012429.4648370
Produced by FastQC (version 0.11.5)