FastQCFastQC Report
Wed 8 May 2024
EGAF00005760470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948179
Total Bases71.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA34280.36153511098642765No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30340.3199817755930051No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC28330.29878324662326416No Hit
GTATGGTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCA27880.2940373073016804No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26050.274737154060573No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25000.2636632956435441No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGCTCACTTTCGGCGGAGGGA24510.2584954950489306No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24170.25490967422817845No Hit
GTGTATTACTGTGCGAGACATTTCGGGTCTGGATACAGCTATGGTTGGTT23180.24446860772069412No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCT22850.24098825221819928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22240.23455486780449686No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22130.23339474930366522No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20550.21673122901899328No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20420.2153601798816468No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGT20130.21230168565218172No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT19370.20428632146461795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19100.20143875787166768No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18900.19932945150651932No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18860.19890759023348967No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18450.19458351218493555No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18230.19226327518327235No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18120.19110315668244077No Hit
GTCACTGGTGCCTCCATCGATACTTACTACTGGAGCTGGATCCGACAGCC17650.18614628672434214No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17590.18551349481479762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17030.17960743699238224No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16450.17349044853345202No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGA16290.1718030034413333No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16280.17169753812307592No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16240.17127567685004624No Hit
CACCTGCACTGTCACTGGTGCCTCCATCGATACTTACTACTGGAGCTGGA16110.16990462771269982No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15950.16821718262058113No Hit
GTCAGAGGGTTAGCAGCAACTTCTTAGCCTGGTACCAGCAGAAACCTGGC15600.1645258964815715No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15540.16389310457202702No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG15450.16294391670771025No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15340.16178379820687866No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15240.16072914502430447No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC14370.15155366233590914No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13750.14501481260394924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13500.14237817964751381No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13460.14195631837448414No Hit
GTATTACTGTGCGAGACATTTCGGGTCTGGATACAGCTATGGTTGGTTTG13050.13763224032593002No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12750.1344682807782075No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA12670.13362455823214814No Hit
CTCCTGCTGGGCCAGTCAGAGGGTTAGCAGCAACTTCTTAGCCTGGTACC12660.13351909291389072No Hit
GTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGA12390.13067152932094045No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG12000.12655838190890117No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGCTCACTTTCGGCGGAGG12000.12655838190890117No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA11860.12508186745329733No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11810.12455454086201025No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11670.12307802640640639No Hit
GGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCACTGG11640.12276163045163413No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11610.12244523449686187No Hit
CTATAACACTGGGAGCACCAACTACAACCCCTCCCTCAACAGTCGAGTCA11480.12107418535951545No Hit
GTCCCTCACCTGCACTGTCACTGGTGCCTCCATCGATACTTACTACTGGA11050.1165391766744465No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11040.11643371135618907No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG10940.1153790581736149No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10850.11442987030929813No Hit
GGATACAGCTATGGTTGGTTTGCCTCCTGGGGCCAGGGAACCCTGGTCAC10810.11400800903626847No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10800.11390254371801106No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGA10780.11369161308149621No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGC10700.11284789053543688No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC10680.11263695989892203No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGA10650.1123205639441498No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10500.1107385841702885No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10430.11000032694248658No Hit
GTGAGGTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCC10340.10905113907816984No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC10320.108840208441655No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC10300.10862927780514017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10180.10736369398605117No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAGGCCTTCGG10170.10725822866779373No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC10120.10673090207650665No Hit
CTACAACCCCTCCCTCAACAGTCGAGTCACCATATCAGTAGACACGTCCA10090.10641450612173439No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT10060.10609811016696213No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGT10040.10588717953044732No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC10000.10546531825741765No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9950.10493799166613055No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGCAGAAATTGTGTTGACGCAGTC9930.10472706102961572No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGC9880.10419973443832863No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9750.1028286853009822No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9720.10251228934620996No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9710.10240682402795254No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9630.10156310148189318No Hit
GCTGTATCCAGACCCGAAATGTCTCGCACAGTAATACACGGCCGTGTCTG9540.10061391361757643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)