FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948179
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA30970.32662609064322246No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29030.3061658189012834No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA26500.2794830933821567No Hit
GTATGGTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT26450.27895576679086964No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24430.2576517725028713No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23680.24974187363356495No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGCTCACTTTCGGCGGAGGGA23600.24889815108750563No Hit
GTGTATTACTGTGCGAGACATTTCGGGTCTGGATACAGCTATGGTTGGTT22710.2395117377625955No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22690.23930080712608062No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGCC21800.22991439380117043No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21450.22622310766216083No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20460.2157820411546765No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGCCCCAGATCCACTGCCA18980.20017317405257867No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18060.19047036477289625No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17810.18783373181646082No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGGGTTAGCAG17470.1842479109957086No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17180.1811894167662435No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17150.18087302081147125No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17040.17971290231063966No Hit
GTCACTGGTGCCTCCATCGATACTTACTACTGGAGCTGGATCCGACAGCC17020.17950197167412482No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16510.17412324044299654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15950.16821718262058113No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15740.16600241093717535No Hit
CACCTGCACTGTCACTGGTGCCTCCATCGATACTTACTACTGGAGCTGGATCCGACAGCCCCCAGGGAAGGGA15390.16231112479816576No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15350.16188926352513608No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15290.16125647161559156No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15270.16104554097907672No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA15190.1602018184330174No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15100.15925263056870065No Hit
GTCAGAGGGTTAGCAGCAACTTCTTAGCCTGGTACCAGCAGAAACCTGGC15030.1585143733408987No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGATCTGGGGCAGACTTCA14720.15524494847491876No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14550.15345203806454266No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14360.15144819701765172No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14080.14849516810644403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14070.1483897027881866No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14040.14807330683341435No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC13500.14237817964751381No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12930.13636665650684102No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12490.13172618250351462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12310.1298278067748811No Hit
GTATTACTGTGCGAGACATTTCGGGTCTGGATACAGCTATGGTTGGTTTGCCTCCTGGGGCCAGGGAACCCTGGT12210.12877315359230695No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12080.1274021044549605No Hit
CTCCTGCTGGGCCAGTCAGAGGGTTAGCAGCAACTTCTTAGCCTGGTACC11970.12624198595412892No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG11660.12297256108814895No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11650.12286709576989155No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAA11450.1207577894047432No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11120.11727743390224843No Hit
GTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGA11070.11675010731096132No Hit
CTATAACACTGGGAGCACCAACTACAACCCCTCCCTCAACAGTCGAGTCACCATATCAGTAGACACGTCCAAGAA11010.11611731540141682No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10930.11527359285535747No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10780.11369161308149621No Hit
GTCCCTCACCTGCACTGTCACTGGTGCCTCCATCGATACTTACTACTGGA10640.11221509862589236No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGA10530.11105498012506076No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG10470.11042218821551628No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10360.10926206971468466No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10350.10915660439642726No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC10340.10905113907816984No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10170.10725822866779373No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10150.10704729803127891No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGCCCCAGATCCACTGCCA10140.10694183271302148No Hit
GTGAGGTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGT10120.10673090207650665No Hit
GGATACAGCTATGGTTGGTTTGCCTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGC9900.10441066507484346No Hit
CTACAACCCCTCCCTCAACAGTCGAGTCACCATATCAGTAGACACGTCCA9820.10356694252878414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9820.10356694252878414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9780.10314508125575444No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGAAGTTGCTGCTAACCCTCTGA9640.10166856680015061No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG9620.10145763616363576No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9600.10124670552712092No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC9540.10061391361757643No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA9490.10008658702628934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG353.2147364E-759.3322340
GTATCAA15950.042.449961
TACACCG503.8121598E-641.4471245
TACTGAT10450.041.34175570
CTCGTAT557.2404946E-637.75088539
ACGGACT753.979403E-836.84188527
TATCAAC18400.036.4164052
CGTATGC601.3099052E-534.62327641
ATCAACG19400.034.539273
GGTATCA8450.034.340331
CAACGCA19900.033.8450135
TCAACGC19850.033.7562644
AACGCAG20200.033.3423656
GCCGATT2353.6379788E-1232.44215470
TTCGGGG13800.031.76627570
GACGGAC901.9747677E-730.70157226
CGCAGAG22000.030.4509288
AGAGTAC21900.030.27461611
CAGTTCG9300.030.05840970
ACGAACC1052.1709639E-829.78734252
Produced by FastQC (version 0.11.5)