FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948179
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29920.3155522322261936No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA29670.31291559926975815No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27080.28560008184108693No Hit
GTATGGTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT24870.26229224650619765No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA24830.261870385233168No Hit
GTGTATTACTGTGCGAGACATTTCGGGTCTGGATACAGCTATGGTTGGTT23390.24668337940409987No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGCTCACTTTCGGCGGAGGGA21930.23128544293851688No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGCC21910.23107451230200202No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21710.2289652059368537No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21520.22696136488996277No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20430.21546564519990424No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19060.20101689659863803No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18880.1991185208700045No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18380.19384525495713362No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGCCCCAGATCCACTGCCA18340.19342339368410394No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18120.19110315668244077No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16630.17538882426208555No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGGGTTAGCAG16330.17222486471436302No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16200.17085381557701657No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15830.16695159880149213No Hit
GTCACTGGTGCCTCCATCGATACTTACTACTGGAGCTGGATCCGACAGCC15760.1662133415736902No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15540.16389310457202702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15490.16336577798073992No Hit
CACCTGCACTGTCACTGGTGCCTCCATCGATACTTACTACTGGAGCTGGATCCGACAGCCCCCAGGGAAGGGA15480.1632603126624825No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14990.15809251206786906No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14840.15651053229400777No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14780.15587774038446328No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14700.15503401783840395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14060.1482842374699292No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14010.1477569108786421No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13940.1470186536508402No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA13850.14606946578652344No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13790.14543667387697892No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.1429055062388009No Hit
GTCAGAGGGTTAGCAGCAACTTCTTAGCCTGGTACCAGCAGAAACCTGGC13390.1412180611466822No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGATCTGGGGCAGACTTCA13380.1411125958284248No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12540.1322535090948017No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12540.1322535090948017No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12500.13183164782177206No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12480.13162071718525722No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12450.13130432123048497No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12180.1284567576375347No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC12150.12814036168276244No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG12090.12750756977321792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11990.12645291659064375No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11940.12592558999935666No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAA11770.12413267958898055No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11560.1219179079055748No Hit
GTATTACTGTGCGAGACATTTCGGGTCTGGATACAGCTATGGTTGGTTTGCCTCCTGGGGCCAGGGAACCCTGGT11430.12054685876822836No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11280.1189648789943671No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11190.11801569113005032No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11130.11738289922050583No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGCAGAAATTGTGTTGACGCAGTC10990.11590638476490199No Hit
GTGAGGTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGT10850.11442987030929813No Hit
CTCCTGCTGGGCCAGTCAGAGGGTTAGCAGCAACTTCTTAGCCTGGTACC10800.11390254371801106No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGA10700.11284789053543688No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG10620.11200416798937754No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGCCCCAGATCCACTGCCA10530.11105498012506076No Hit
GTCCCTCACCTGCACTGTCACTGGTGCCTCCATCGATACTTACTACTGGA10470.11042218821551628No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10450.11021125757900144No Hit
CTATAACACTGGGAGCACCAACTACAACCCCTCCCTCAACAGTCGAGTCACCATATCAGTAGACACGTCCAAGAA10420.10989486162422918No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10220.10778555525908083No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10200.10757462462256599No Hit
GTACCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGA10160.10715276334953633No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10120.10673090207650665No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10070.10620357548521955No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10030.10578171421218989No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC9920.10462159571135829No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9770.10303961593749703No Hit
GGACTGGAGTGGATTGGCTATTTCTATAACACTGGGAGCACCAACTACAA9610.10135217084537836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC150.002360250668.964358
TACTGAT9500.045.63453770
GTATCAA16800.041.35361
TATCAAC18850.036.6419032
ATCAACG20550.033.5773
TCAACGC20600.033.4778374
CTACTGA9150.033.1544269
AACGCAG21700.032.0986146
CAACGCA21700.031.939715
CTATTAA450.004426163630.93962755
CGTTGAA1151.6370905E-930.10994542
GTCTACT8750.029.83311767
GGTATCA9900.029.6763341
TGTCTAC8750.029.53589866
AATAGCG952.8536306E-729.4381662
TCTACTG9300.029.2960768
CGCAGAG23950.029.0830868
AGAGTAC23950.028.93911211
GCAGAGT24200.028.7826429
TAGAGTA500.007753776427.585745
Produced by FastQC (version 0.11.5)