FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769894
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGCC81660.4613835630834389No Hit
GTATAATAGTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT73160.4133580881114914No Hit
GTAATATACAGCCGTGTCTGTGGCGGTCACAGAGCTCAGCCTCAGGGAGA56440.31888915381373123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55220.311996085641287No Hit
GTCTCTGGTGGCTCCGTCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG46130.26063707770069844No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42940.2426133994465205No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41720.2357203312740763No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC41640.2352683268037521No Hit
GTATATTACTGTGCGAGACATCATAGCAGCTCGTCCAGATATTTTCCGTT40160.22690624410275417No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37830.21374161390456153No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC35100.1983169613547478No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34820.19673494570861305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34770.1964524429146604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33830.19114139038835093No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC33590.1897853769773783No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33570.18967237585979727No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33550.1895593747422162No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA33400.1887118663603583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32850.18560433562687934No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31830.17984127863024565No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31430.1775812562786246No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31000.17515173225063196No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30360.17153569648803826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29970.16933217469520773No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28820.1628346104342972No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28210.1593880763480751No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG28180.1592185746717035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28140.1589925724365414No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27830.15724105511403508No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAAATGTGACAT27630.15611104393822456No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25450.1437939221218898No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25250.14266391094607925No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25180.14226840703454557No Hit
ACCTACTACCACCCGTCCCTCAAGAGTCGACTCACCATATCCGTAGACACGTCCAAGACCCAGTTCTCCCTGAGG24930.1408558930647824No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA24650.1392738774186477No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24210.1367878528318645No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGA23370.1320418058934603No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23260.1314202997467645No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22990.12989478465942028No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22510.127182757837475No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21950.12401872654520553No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21850.12345372095730026No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21650.12232370978148974No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT21150.11949868184196343No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20980.11853817234252448No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20930.11825566954857182No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20890.11802966731340972No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT20850.11780366507824762No Hit
GTAGTAGGTACTCCCACTATAAGAGATACTCCCAATCCACTCCAGTCCCTTTCCTGGGGGCTGGCGGATCCAGCC20580.11627814999090341No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20490.11576964496178867No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20180.11401812763928235No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20110.11362262372774867No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC20060.11334012093379604No Hit
CAGCTGCACTGTCTCTGGTGGCTCCGTCAGCAGTAGTAGTTACTACTGGG19990.11294461702226234No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19600.11074109522943182No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19400.10961108405362129No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19210.10853757343660128No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAT19170.10831157120143918No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19150.10819857008385814No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19010.10740756226079076No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18950.1070685589080476No Hit
GAGTGAATTCTGCCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA18880.1066730549965139No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18870.10661655443772339No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT18810.10627755108498023No Hit
ACCTTGATGGGACCCCAGTTTCTAAACTCGACGCCTTATTGATCAGTAACTTAGGGGCTTTCCCTGGTTTCTG18040.1019270080581097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA200.007390607651.69514527
TACGGAT20800.044.60259670
GTATCAA28950.042.627641
TATCAAC33150.037.307612
GTGGTAT5050.036.177731
TCAACGC34350.035.8664634
ATCAACG34400.035.7253953
CAACGCA35600.034.607115
AACGCAG36000.034.2235536
AGAGTAC38200.032.43641311
CGCAGAG38700.031.924798
GCAGAGT40100.030.9818539
AATATAC18050.029.3704643
GTAATAT18200.029.3573681
TAATATA18300.028.9905282
TATTACG500.00631232428.77400267
CTACGGA21650.028.61737469
ACGCAGA43650.028.225617
TATACAG19650.028.0213955
ATATACA19450.027.601794
Produced by FastQC (version 0.11.5)