FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616284
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61500.036.6469881
GGTATCA28850.031.6514341
ATCAACG72750.031.0650393
TCAACGC73000.031.006084
CAACGCA75250.030.2158785
AACGCAG76700.029.6892286
TATCAAC78200.028.9880182
ACGCAGA82800.027.5819327
CGCAGAG84900.026.8594238
GTGGTAT13200.026.1682931
AGAGTAC85500.026.15011411
CAGAGTA86900.025.8473410
TGGTATC14300.025.1052762
GCAGAGT94100.024.1602979
AGTACGG72800.023.76144213
GAGTACG74150.023.28272812
GTACGGG87050.019.7931714
GTAGTTT11350.019.40868670
CGTATGC1258.4888394E-519.39533841
TACGGGG35500.018.5858615
Produced by FastQC (version 0.11.5)