FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616284
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69600.033.888411
GGTATCA34500.031.3551731
AACGCAG80750.029.0680756
TCAACGC80750.028.8993824
ATCAACG81200.028.8316443
CAACGCA82200.028.3474885
TATCAAC83150.028.3053232
GTGGTAT16250.027.8074871
ACGCAGA86050.027.2380057
CGCAGAG89350.026.1560348
AGAGTAC89250.025.84326611
CAGAGTA89600.025.74034710
TGGTATC17950.024.3942492
GCAGAGT96600.024.2637169
AGTACGG81750.022.9937513
GAGTACG83850.022.41573512
CCGTTAA4850.021.84722916
GCCGTTA5100.021.4452615
GTACGGG96550.019.3282214
TTTAGCG2709.094947E-1219.00579326
Produced by FastQC (version 0.11.5)