FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488925
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA44450.29853753547022177No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28290.19000285440838188No Hit
GTGTATTACTGTGCGAGATCTCCCCTTAGTATGACCGCCGTTCTCTTCCG27250.1830179491915308No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26980.18120456033715598No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25640.17220477861544403No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24520.16468257299729672No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24360.16360797219470424No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG23770.15964538173514448No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23630.15870510603287608No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23310.1565559044276911No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22810.15319777691958963No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20620.13848917843410513No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG20310.13640713937908222No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA19130.12848195845996271No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC18920.1270715449065601No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT18210.12230300384505599No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC17980.12075826519132932No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17490.11746730023338987No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17340.11645986198095942No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17010.11424349782561244No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGT16800.11283308427220981No Hit
CTCCTGTGAAGTCTCTGGATTCACCCTCAGTAGCTTTTGGATGAGCTGGGTCCGCCAGGCTCCCGGGAAGGGG16690.11209429622042748No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16670.11195997112010342No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16570.11128834561848312No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16010.10752724280940947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15970.10725859260876135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.10544520375438655No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15690.10537804120422452No Hit
CCCTGAGACTCTCCTGTGAAGTCTCTGGATTCACCCTCAGTAGCTTTTGG15090.10134828819450274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28300.040.4059141
TCGTATG655.2927135E-737.2953340
TATCAAC32600.034.8561632
ACGCGTA601.3308045E-534.54528427
ATCAACG32900.034.5383223
TCAACGC33000.034.3301544
CAACGCA34000.033.422035
AACGCAG34050.033.2703976
GTGGTAT3550.032.1135981
CGCGTAG652.2996666E-531.89116928
GACGCGT652.3023089E-531.88581326
CGTATGC802.6760954E-630.31062341
CGCAGAG37850.030.1116628
AGAGTAC37650.029.99339911
CAGAGTA37950.029.75729610
ATTCGCG605.367753E-428.80031630
ACGCAGA39800.028.4618327
GCAGAGT40600.027.9851329
GGTATCA20000.027.982621
TTCGGGG14350.024.85488170
Produced by FastQC (version 0.11.5)