FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488925
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA39130.262807058784022No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33540.22526319324344743No Hit
GTGTATTACTGTGCGAGATCTCCCCTTAGTATGACCGCCGTTCTCTTCCG27420.18415971254428531No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26230.17616736907500377No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24280.163070671793408No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23860.16024984468660275No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22670.15225750121732123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22490.1510485753144047No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG21930.14728747250533103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21010.1411085178904243No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20890.14030256728847992No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG19090.1282133082593146No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18510.12431788034991688No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGT18260.12263881659586615No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17700.11887771378679247No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17450.11719865003274174No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17330.11639269943079739No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17290.11612404923014928No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT17200.115519586278691No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17130.1150494484275568No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16960.1139076850748023No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16800.11283308427220981No Hit
CTCCTGTGAAGTCTCTGGATTCACCCTCAGTAGCTTTTGGATGAGCTGGGTCCGCCAGGCTCCCGGGAAGGGG16130.10833319341135383No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC16060.10786305556021963No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15750.10578101650519671No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15480.10396762765082192No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15410.10349748979968769No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15120.10154977584498884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.042.9728621
GTATCAA29550.039.9831161
TATCAAC33400.035.3445432
ATCAACG35450.033.1731573
TCAACGC35700.033.122924
CAACGCA36500.032.4924855
AACGCAG37350.031.8453376
GTAGTTT3250.031.21467870
AGAGTAC39350.030.14320411
CGCAGAG39800.029.7993838
CAGAGTA40700.029.2251410
TCGTTTA604.965826E-429.1858362
ACGCAGA41200.028.8694957
GCAGAGT42700.027.9370259
GGTATCA20000.027.4647161
CGAACTA1404.638423E-1027.12475629
TCTGGAG22150.024.90392370
AGTACGG37000.023.95732913
GAGTACG37750.023.47898912
TGTTAGA5150.023.39206970
Produced by FastQC (version 0.11.5)