FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784359
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19100.24351094333079623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18120.2310166645630381No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA11580.14763647768432567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11470.14623405863896508No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10750.13705458852387745No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9950.12685517728489124No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9940.1267276846444039No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9820.12519777295855597No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9160.11678325868639233No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9110.11614579548395569No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATT9100.11601830284346835No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGCGGGTCTGGGACAGAATTCACT8920.11372343531469646No Hit
CCCTATCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCTGGGCCAGTGAGACTATTAGCATCAACCTAGC8390.10696632536886808No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCAGAGAAGTCAACTTCAGGGAGA8310.10594638424496945No Hit
GTCTCCAGCCACCCTATCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCTGGGCCAGTGAGACTATTAGCAT8180.1042889799186342No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8160.10403399463765955No Hit
TCCATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGCGGGTCTGGGACAGAATTCACT8100.10326903879473559No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7920.10097417126596368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.041.2452281
GTAGTTT2450.041.16719470
GTATCAA17300.040.4296881
CCAACCG1400.037.12422643
GTGGTAT2300.035.9528961
TCGTATG601.31380675E-534.60711740
TATCAAC20400.034.2859572
ATCAACG20550.034.0356983
TCAACGC20750.033.707644
CGAAATT1550.033.61778648
CAACGCA20950.033.3858535
AACGCAG21150.033.070156
TCGCCCC1650.031.426838
CGCAGAG22600.030.9444438
CCGAAAT1700.030.62394747
AGAGTAC23300.030.01286711
ACGCAGA23650.029.5705877
AACCGAA1800.028.90034345
CGGGATA854.477779E-628.3691617
CAGAGTA25150.027.94213110
Produced by FastQC (version 0.11.5)