FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254062
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27370.12142523142664222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65700.038.6573371
GTGGTAT12450.038.094531
TATCAAC75750.033.4616052
ATCAACG76850.032.825253
TCAACGC77300.032.4923024
GGTATCA29550.032.4488681
CAACGCA78050.032.180085
TGGTATC14450.032.087732
AACGCAG81100.031.0121616
ACGCAGA85150.029.5371277
CGCAGAG87900.028.5740058
AGAGTAC88600.028.42759911
CAGAGTA89550.028.04689410
GCAGAGT95100.026.5177389
AGTACGG71900.025.15403713
GAGTACG72850.024.82436612
GTACGGG84200.021.3591414
TACGGGG41750.020.30554615
AGTACTT35150.019.91731813
GTACTTT35400.019.58450714
Produced by FastQC (version 0.11.5)