FastQCFastQC Report
Wed 8 May 2024
EGAF00005760482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905370
Total Bases67.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATCGTTATCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTA33750.3727757712316511No Hit
CGATAATACTGTTCGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCT28240.3119166749505727No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27290.301423727315904No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20420.22554314810519457No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18710.2066558423627909No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18340.20256911538928837No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17580.1941747572815534No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17390.19207616775461966No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17320.1913030031920651No Hit
AGTCAGGACATTAGCAATTATGTAGCCTGGTTTCAGCAGAAACCTGGGAA16260.17959508267338214No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16230.17926372643228736No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA15950.1761710681820692No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15590.1721947932889316No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15400.17009620376199786No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15160.16744535383323944No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15110.16689309343141479No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14660.16192274981499274No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG13950.15408065210908248No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13880.15330748754652795No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGTCGGGCGAGTCAGG13520.14933121265339033No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCT13460.14866850017120073No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT13390.14789533560864618No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13330.14723262312645657No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT13220.14601765024244232No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13150.14524448567988776No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13040.1440295127958735No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT12950.1430354440725891No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12910.14259363575112938No Hit
GTTTATTACTGTGCGAGGAAGTCGGCCTATTTCTATGACAGTAGTGGTTA12870.14215182742966964No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT12820.14159956702784496No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC12650.1397218816616411No Hit
GATCTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCG12160.13430972972375935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11880.1312170714735412No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11870.13110661939317625No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11550.1275721528214984No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG11260.12436904249091532No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGCGAAC10930.12072412383887249No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10870.12006141135668291No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCC10810.11939869887449331No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10460.11553287606172064No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10350.11431790317770635No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC10270.11343428653478689No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC10250.11321338237405701No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10190.1125506698918674No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9990.11034162828456875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9680.1069176137932558No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA9680.1069176137932558No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC9660.10669670963252592No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA9660.10669670963252592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)