FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905370
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATCGTTATCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT32350.3573124799805604No Hit
CGATAATACTGTTCGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC27070.2989937815478755No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26270.29015761511868077No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC18270.20179595082673382No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16630.18368180964688471No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16480.1820250284414107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15990.17661287650352894No Hit
AGTCAGGACATTAGCAATTATGTAGCCTGGTTTCAGCAGAAACCTGGGAA15880.17539790361951468No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15670.17307840993185106No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA15180.16766625799396934No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14750.16291681853827716No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14680.16214365397572264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14590.16114958525243825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14450.15960325612732915No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14350.15849873532367983No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13890.15341793962689287No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13880.15330748754652795No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13500.14911030849266044No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGTCGGGCGAGTCAGG13330.14723262312645657No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12870.14215182742966964No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT12780.14115775870638525No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG12780.14115775870638525No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12570.13883826501872162No Hit
GTTTATTACTGTGCGAGGAAGTCGGCCTATTTCTATGACAGTAGTGGTTA12510.13817555253653202No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT12480.13784419629543723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12460.13762329213470736No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT12370.13662922341142297No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT12090.13353656516120482No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12070.13331566100047496No Hit
GATCTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA11680.12900802986624255No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11240.12414813833018544No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC11240.12414813833018544No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10910.12050321967814265No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGCGAACAGTATTATCGTTATCCGGTCACCTT10560.11663739686536996No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA10290.11365519069551674No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10220.11288202613296222No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG10030.11078343660602848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9910.10945801164164927No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9870.10901620332018955No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC9810.10835349083799994No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9760.10780123043617527No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9620.1062549013110662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9310.10283088681975325No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9290.1026099826590234No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9240.10205772225719871No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA9180.1013950097750091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2350.041.1393241
GGTATCA7000.040.9399221
GTATCAA17100.040.7870451
TATCAAC19250.036.0482642
CTCGTAT400.002551718634.62136539
ATCAACG20250.034.438593
TCAACGC20650.033.77154
CAACGCA21100.033.0512545
AACGCAG21150.032.9731186
TGGTATC3100.031.1828172
CGTATGC450.00452255230.80355541
CGCAGAG23600.029.3989038
ACGCAGA24300.028.9781727
CAGAGTA23950.028.96927310
AGAGTAC23800.028.86178611
GCAGAGT24800.027.8371899
ACGAGAC500.007699738327.6251722
CGGTACC1057.2952025E-726.44116245
CGCGGTA1057.351973E-726.41769243
TGTTAGA3400.025.7747670
Produced by FastQC (version 0.11.5)