FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570971
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27500.040.249221
TCAACGC30550.036.225124
ATCAACG31300.035.4664653
CAACGCA31400.035.3546455
TATCAAC31450.035.2973142
AACGCAG31650.034.966116
GGTATCA12850.033.3778841
ACGCAGA35100.031.6247837
AGAGTAC36650.029.81459211
CGCAGAG37300.029.7595148
CAGAGTA39150.028.26492310
CGTTAAC500.007646053627.6660583
GCAGAGT40800.027.2913689
TTAACGG2950.024.64320235
TTTAACG3050.023.83067134
AGTACGG34100.023.22185513
TAACGGC2850.023.08149536
GAGTACG35300.022.53040112
GTATATA1854.6929927E-1022.4362221
GTATTAG4950.021.6619071
Produced by FastQC (version 0.11.5)