FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672058
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26280.1571715813685889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49250.038.4220961
GGTATCA20850.034.8177721
TATCAAC57150.032.904672
ATCAACG58050.032.438213
TCAACGC58300.032.1716084
CAACGCA59150.031.7664185
AACGCAG59950.031.4571086
GTGGTAT9550.030.982731
ACGCAGA63450.029.7760247
CGCAGAG65550.028.8745048
CAGAGTA65900.028.35711910
AGAGTAC65800.028.34801111
TGGTATC10350.027.9013612
GCAGAGT70700.026.8683729
AGTACGG57450.024.8795713
GAGTACG57950.024.66121712
ACGGGTA3400.024.25698716
GTACGGG66700.021.27535214
TACGGGT9100.021.14648415
GAGTACT21300.020.48324412
Produced by FastQC (version 0.11.5)