FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510015
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62140.4115190908699582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43840.29032824177243277No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41750.27648731966238743No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40290.2668185415376668No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA39720.26304374459856356No Hit
GTCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT39440.2611894583828637No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG37710.24973261855014686No Hit
GTAATACATGGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAAATACA37690.2496001695347397No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37130.24589159710334002No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCACTTTTTTAGCCTGGTATCAGCAAAAA36540.24198435114882966No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC36540.24198435114882966No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCA35770.2368850640556551No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35600.23575924742469445No Hit
ACCTTGGACCCTCCGCCGAAACCCCGAGGGAAACTATTAAGCTGTTGACAATAATAAGTTGCACAATCTTCAGG35280.23364006317818034No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35170.23291159359344113No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34630.22933547017744857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34550.22880567411582003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34330.22734873494634159No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT33490.22178587629924207No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33450.22152097826842782No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33350.22085873319139215No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC33280.22039516163746717No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGACCCTCCGCCGAAACCC33270.22032893712976362No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33200.21986536557583863No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33050.21887199796028514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31560.20900454631245383No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31050.205627096419572No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29950.19834240057217972No Hit
CTATTAAGCTGTTGACAATAATAAGTTGCACAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC29800.19734903295662629No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29310.19410403207915153No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG28370.18787892835501635No Hit
ATGTATTACTGCGCGAGAGATCAAGATATTTTGACTGCTTTTTACTCGCCGTTTGACTCCTGGGGCCAGGGAACC28170.18655443820094503No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC26660.17655453753770659No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26640.17642208852229946No Hit
GCTTAATAGTTTCCCTCGGGGTTTCGGCGGAGGGTCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26530.17569361893756022No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAATAACTATTGG25760.17059433184438566No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25490.16880627013638938No Hit
ATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC24450.1619189213352185No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24350.16125667625818285No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24070.15940239004248302No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23440.15523024605715838No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23040.15258126574901576No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22950.15198524517968365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22750.15066075502561233No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA22690.15026340797939094No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22530.14920381585613388No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG22500.14900514233302317No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22420.14847534627139464No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22390.14827667274828396No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21860.14476677383999495No Hit
CAGTAATACATGGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAAATA21830.14456810031688427No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21310.14112442591629884No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTAT21170.14019728280844893No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20910.1384754456081562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGACC20380.1349655466998672No Hit
GCCTGAAGATTGTGCAACTTATTATTGTCAACAGCTTAATAGTTTCCCTC20220.13390595457661017No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20140.13337615851498164No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20040.13271391343794597No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC20020.13258146442253885No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19990.13238279089942817No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19810.13119074976076397No Hit
GTCAGGGCATTAGCACTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTG19710.1305285046837283No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA19570.12960136157587837No Hit
CTCCAGAGACAACGCCAAAAACTCACTGTATTTGCAAATGAACAGCCTGAGAGCCGGGGACACGGCCATGTATTA19390.1284093204372142No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19330.1280119733909928No Hit
GTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAAGTGCTAATG19010.12589278914447868No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18940.12542921759055373No Hit
GGGTTCCCTGGCCCCAGGAGTCAAACGGCGAGTAAAAAGCAGTCAAAATA18810.12456829899040738No Hit
GTATTACTGCGCGAGAGATCAAGATATTTTGACTGCTTTTTACTCGCCGTTTGACTCCTGGGGCCAGGGAACC18770.12430340095959311No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18640.12344248235944676No Hit
GTGTGGGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTAAGACTCTCCTGTGCAGC18300.12119084909752553No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAATAACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18250.12085972655900769No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18110.11993258345115777No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTGTGCAACTTATTATTGTCAACAGCTTAATAGTTTC18070.1196676854203435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17810.1179458482200508No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA17800.11787962371234723No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17700.11721737863531158No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGGAAGATGGAAATGAGAAATACTA17630.1167538070813866No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC17590.11648890905057235No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17520.11602533749664738No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC17440.11549554143501885No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17330.11476707185027962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17300.11456839832716893No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17180.11377370423472616No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT17060.11297901014228336No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17040.11284656112687623No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA16930.112118091542137No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16770.11105849941887994No Hit
ACATAAAGGAAGATGGAAATGAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG16690.11052870335725143No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16590.10986645828021575No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC16560.10966778475710506No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16550.1096015602494015No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16510.10933666221858723No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16510.10933666221858723No Hit
ATACATGGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAAATACAGTG16380.10847574361844087No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA16330.10814462107992305No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT16180.10715125346436956No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC16070.10642278387963033No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTGTG15840.10489962020244832No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC15750.10430359963311622No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG15740.10423737512541267No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG15540.10291288497134134No Hit
CCTTGGACCCTCCGCCGAAACCCCGAGGGAAACTATTAAGCTGTTGACAATAATAAGTTGCACAATCTTCAGG15470.10244931341741638No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA15380.1018532928480843No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG15230.1008599252325308No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15210.10072747621712368No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG15210.10072747621712368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG200.006823004752.75007265
GTGGTAT2300.043.506431
GTATCAA22650.041.131911
GGGCGTA451.0020149E-438.331385
CGAAAGA700.002068516636.13463670
TATCAAC26200.035.4199522
CCTCGTT901.6729491E-435.13089870
ATCAACG27400.033.8687173
TCAACGC27900.033.261754
TCTGGAG17550.032.78883770
CAACGCA28750.032.278365
AACGCAG29500.031.4577226
GTTGAAA24700.029.69769770
CGCAGAG31950.029.0425838
AGAGTAC32150.028.86191711
AATATTC605.314119E-428.84980841
CAGAGTA32650.028.41992610
GCAGAGT32950.028.265869
TTCGGGG19450.027.9602270
ACGGACT500.007621992427.68385540
Produced by FastQC (version 0.11.5)