FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311887
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73950.037.959641
GGTATCA32700.035.261311
TATCAAC86200.032.755692
TCAACGC87600.032.3105284
ATCAACG88150.032.0700073
CAACGCA90050.031.5464275
AACGCAG91050.031.1239196
ACGCAGA96900.029.2430237
CGCAGAG101800.027.8354538
AGAGTAC100250.027.75192311
CAGAGTA101600.027.48446710
GTGGTAT11200.027.2696081
GCAGAGT107200.026.368729
TCGTATG2450.025.44188940
AGTACGG77700.024.37054413
GAGTACG78450.024.05010212
TGGTATC13800.023.866372
CGTATGC2550.023.10886241
ACGGGTA3450.022.86946516
TACGGGG34300.021.30260515
Produced by FastQC (version 0.11.5)